GUS reporter activity measurement
Freshly harvested whole seedlings or leaf tissue is incubated in a multi-well polypropylene plate containing absolute heptane for 10 minutes at ambient room temperature. Following removal of the heptane, plant tissue is air dried for 5 min in a flow hood.
Plants are then immersed in GUS solution (1mM X-Gluc (Roth), 0.5mM potassium ferrocyanide, 0.1% (v/v) triton X-100, 100mM sodium phosphate buffer pH 7.0), and incubated at 37°C overnight. The following day the X-Gluc solution should be discarded. The tissue is washed repeatedly with 70% ethanol to remove chlorophyll. This enables the GUS stain to be visualised.